Hormonal Regulation of the Phosphoenolpyruvate Carboxykinase Gene

Multihormonal Regulation of Phosphoenolpyruvate Carboxykinase Gene Transcription

We used a nuclear RNA transcript elongation assay to show that cAMP analogs and dexamethasone cause a selective increase of transcription of the P-enolpyruvate carboxykinase gene in H4IIE hepatoma cells. 8-(4-~hlorophenylthio)-cAMP increased transcription within 5 min and the maximal rate, generally 10-15fold above the basal rate, was attained by 30 min. This increase was of sufficient magnitud...

PPARg2 Regulates Adipose Expression of the Phosphoenolpyruvate Carboxykinase Gene

Aspects of the control of phosphoenolpyruvate carboxykinase gene transcription.

Published, JBC Papers in Press, July 27, 2009, DOI 10.1074/jbc.R109.040535 Jianqi Yang, Lea Reshef, Hanoch Cassuto, Gabriela Aleman, and Richard W. Hanson From the Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4936 and the Department of Biochemistry and Molecular Biology, Hebrew University-Hadassah School of Medicine, Jerusalem 991120, Israel

Regulation of the acuF gene, encoding phosphoenolpyruvate carboxykinase in the filamentous fungus Aspergillus nidulans.

Phosphoenolpyruvate carboxykinase (PEPCK) is a key enzyme required for gluconeogenesis when microorganisms grow on carbon sources metabolized via the tricarboxylic acid (TCA) cycle. Aspergillus nidulans acuF mutants isolated by their inability to use acetate as a carbon source specifically lack PEPCK. The acuF gene has been cloned and shown to encode a protein with high similarity to PEPCK from...

Hormonal regulation of chimeric genes containing the phosphoenolpyruvate carboxykinase promoter regulatory region in hepatoma cells infected by murine retroviruses.

Hepatoma cells were infected with replication-incompetent murine retroviruses containing the selectable gene for amino-3'-glycosyl phosphotransferase (neo) and/or the nonselectable gene for bovine growth hormone (bGH). Expression of these genes was controlled by the promoter regulatory region of the gene for the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK) fro...